The open‐access high‐throughput crystallization facility at EMBL Hamburg
Identifieur interne : 000134 ( OpenAccess/Analysis ); précédent : 000133; suivant : 000135The open‐access high‐throughput crystallization facility at EMBL Hamburg
Auteurs : Jochen Mueller-Dieckmann [Allemagne]Source :
- Acta Crystallographica Section D [ 1399-0047 ] ; 2006-12.
English descriptors
Abstract
Here, the establishment of Europe's largest high‐throughput crystallization facility with open access to the general user community is reported. The facility covers every step in the crystallization process from the preparation of crystallization cocktails for initial or customized screens to the setup of hanging‐drop vapour‐diffusion experiments and their automatic imaging. In its first year of operation, 43 internal and 40 external users submitted over 500 samples for a total of 2985 crystallization plates. An electronic booking system for registration, the selection of experimental parameters (e.g. drop size, sample‐to‐reservoir ratio) and the reservation of time slots was developed. External users can choose from more than 1000 initial crystallization conditions. By default, experiments are kept for six months and are imaged 15 times during this time period. A remote viewing system is available to inspect experiments via the internet. Over 100 stock solutions are available for users wishing to design customized screens.
Url:
DOI: 10.1107/S0907444906038121
Affiliations:
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ISTEX:FF7682D61DF5534312D929DD66F3239BAF264F2DLe document en format XML
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